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mouse rabbit ig  (Vector Laboratories)


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    Vector Laboratories mouse rabbit ig
    Mouse Rabbit Ig, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immpress+anti+rabbit+ig+peroxidase+polymer+detection+kit/ImmPRESS+HRP+Universal+Antibody+(anti-mouse+IgG%2Fanti-rabbit+IgG%2C+Peroxidase)+Polymer+Detection+Kit%2C+made+in+Horse/pmc12732205-227-27-29
    Average 96 stars, based on 334 article reviews
    mouse rabbit ig - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymer:

    Article Title: Myeloid ZFP36L1 Does Not Regulate Inflammation or Host Defense in Mouse Models of Acute Bacterial Infection
    Article Snippet: The slides were incubated with an antibody against ZFP36L1 (TISB Ab-92 Assay Biotech #B1184), ZFP36 (Sigma Aldrich #T5327), or a non-targeting antibody against rabbit IgG (Santa Cruz #SC-2027). .. Primary antibodies were then detected using ImmPRESS Anti-Rabbit Ig (peroxidase) Polymer Detection Kit (Vector Laboratories) and ImmPACT DAB Peroxidase Substrate (Vector Laboratories) and stained with Hemotoxylin and Eosin. .. To determine bacterial burden, whole lungs, livers, and spleens were homogenized in 5 ml sterile water using the Next Advance Bullet Blender system.

    Article Title: Discordant growth of nasal cartilage and bone contributes to phenotypic variability of the skull in a mouse model.
    Article Snippet: .. The secondary antibody (ImmPRESS Anti-Rabbit Ig Peroxidase Polymer Detection Kit, Vector Laboratories, Cat. No. MP-6401) for each sample of Osx was labeled with diaminobenzidine (DAB Peroxidase Substrate Kit, 3,30-diaminobenzidine, Vector Laboratories, Cat. No. SK-4100) following standard protocols and counterstained using Methyl Green (Sigma, Cat. No. 67060).17 Osteoclast number was assessed through TRAP staining (Sigma-Aldrich, Cat. No. 387-1KT) as per manufacturer's instructions. ..

    Article Title: Baicalein Protects against Type 2 Diabetes via Promoting Islet β -Cell Function in Obese Diabetic Mice
    Article Snippet: .. Baicalein (98% pure by HPLC) for in vivo studies was purchased from Xi'An Yile Bio-Tech Company, China; ultrasensitive rat insulin enzyme-linked immunosorbent assay (ELISA) kits were obtained from Mercodia (Winston-Salem, NC); the active form of the caspase-3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); the ImmPRESS Anti-rabbit Ig (peroxidase) Polymer Detection kit, Vector NovaRED peroxidase substrate kit, and Vector SG peroxidase substrate kits were from Vector laboratories (Burlingame, CA); cell viability assay kits were from Promega (Madison, WI); and the BrdU ELISA kit for the cell proliferation assay was from Roche Applied Sciences (Indianapolis, IN). ..

    Staining:

    Article Title: Myeloid ZFP36L1 Does Not Regulate Inflammation or Host Defense in Mouse Models of Acute Bacterial Infection
    Article Snippet: The slides were incubated with an antibody against ZFP36L1 (TISB Ab-92 Assay Biotech #B1184), ZFP36 (Sigma Aldrich #T5327), or a non-targeting antibody against rabbit IgG (Santa Cruz #SC-2027). .. Primary antibodies were then detected using ImmPRESS Anti-Rabbit Ig (peroxidase) Polymer Detection Kit (Vector Laboratories) and ImmPACT DAB Peroxidase Substrate (Vector Laboratories) and stained with Hemotoxylin and Eosin. .. To determine bacterial burden, whole lungs, livers, and spleens were homogenized in 5 ml sterile water using the Next Advance Bullet Blender system.

    Article Title: Discordant growth of nasal cartilage and bone contributes to phenotypic variability of the skull in a mouse model.
    Article Snippet: .. The secondary antibody (ImmPRESS Anti-Rabbit Ig Peroxidase Polymer Detection Kit, Vector Laboratories, Cat. No. MP-6401) for each sample of Osx was labeled with diaminobenzidine (DAB Peroxidase Substrate Kit, 3,30-diaminobenzidine, Vector Laboratories, Cat. No. SK-4100) following standard protocols and counterstained using Methyl Green (Sigma, Cat. No. 67060).17 Osteoclast number was assessed through TRAP staining (Sigma-Aldrich, Cat. No. 387-1KT) as per manufacturer's instructions. ..

    other:

    Article Title: wPrognostic significance of VHL, HIF1A, HIF2A, VEGFA and p53 expression in patients with clear-cell renal cell carcinoma treated with sunitinib as first-line treatment
    Article Snippet: Following antigen retrieval in hot (90°C) acidic citrate buffer (Epitope Retrieval Solution, pH 6.0; Leica Biosystems Ltd.), the samples were blocked for endogenous peroxidase activity by incubation with 3% hydrogen peroxide for 10 min, followed by incubation with 2.5% normal horse serum [ImmPRESSTM Anti-Rabbit Ig (peroxidase) Polymer Detection kit, Vector Laboratories Inc.; part of Maravai LifeSciences] to block the non-specific binding of immunoglobulin.

    Article Title: Phytonutrient genistein is a survival factor for pancreatic β-cells via GPR30-mediated mechanism
    Article Snippet: Cell culture medium, supplements, palmitate, fatty acid-free bovine serum albumin (BSA), H89, mellittin, and protease and phosphatase inhibitor cocktails were from Sigma (St. Louis, MO); genistein (purity≥99.0%) was from LC Laboratories (Woburn, MA); ICI 182,780 (ICI), 17β-estradiol (E2), and G15 were from Tocris Biosciences (Bristol, UK); insulin ELISA kits were obtained from Crystal Chemical Inc. (Chicago, IL); cell viability and caspase-3 assay kits, pRL luciferase control vector, and dual luciferase assay system were purchased from Promega (Madison, WI); cyclic AMP EIA kits were from Cayman Chemical Company (Ann Arbor, MI); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kits were from Trevigen Inc. (Gaithersburg,MD); the active form of the caspase −3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); antibodies for phospho-CREB (ser133), CREB, and β-actin were from Cell Signaling Technology (Beverly, MA); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); CREB ShortCut® siRNA, scramble sequence of siRNA and transfection reagents were from New England Biolabs (Ipswich, MA); anti-GPR30, anti-Gαs, shGαs lentiviral particles, and GPR30 siRNA (rats) were from Santa Cruz Biotechnology (Santa Cruz, CA); a reporter plasmid containing a promoter region of the Bcl-2 gene linked to a firefly luciferase gene (Bcl-2-Luc) was a gift of Dr. Linda M. Boxer, Stanford University); a reporter plasmid containing multiple copies of a consensus cAMP responsive element (CRE)-binding sequence fused to a TATA-like promoter region upstream of the gene for firefly luciferase (CRE-luc) was from BD Clontech, CA; the plasmid transfection reagent was from Invitrogen, CA; the ImmPRESS anti-rabbit Ig (peroxidase) polymer detection kit, vector NovaRED peroxidase substrate, and vector SG peroxidase substrate were from Vector Laboratories (Burlingame, CA).

    Article Title: wPrognostic significance of VHL, HIF1A, HIF2A, VEGFA and p53 expression in patients with clear-cell renal cell carcinoma treated with sunitinib as first-line treatment
    Article Snippet: Statistical analysis was performed using GraphPad Prism version 6.07 (GraphPad Software) and Statistica version 13 (Dell Inc.) software.

    Article Title: wPrognostic significance of VHL, HIF1A, HIF2A, VEGFA and p53 expression in patients with clear-cell renal cell carcinoma treated with sunitinib as first-line treatment
    Article Snippet: The slides were rinsed in PBS, and immunoreactive cells were visualized by the addition of 3,3′-diaminobenzidine solution (DAB Peroxidase Substrate kit, Vector Laboratories, Inc.) and counterstained with hematoxylin.

    Article Title: Phytonutrient genistein is a survival factor for pancreatic β-cells via GPR30-mediated mechanism
    Article Snippet: Materials and Reagents Cell culture medium, supplements, palmitate, fatty acid-free bovine serum albumin (BSA), H89, mellittin, and protease and phosphatase inhibitor cocktails were from Sigma (St. Louis, MO); genistein (purity≥99.0%) was from LC Laboratories (Woburn, MA); ICI 182,780 (ICI), 17β-estradiol (E2), and G15 were from Tocris Biosciences (Bristol, UK); insulin ELISA kits were obtained from Crystal Chemical Inc. (Chicago, IL); cell viability and caspase-3 assay kits, pRL luciferase control vector, and dual luciferase assay system were purchased from Promega (Madison, WI); cyclic AMP EIA kits were from Cayman Chemical Company (Ann Arbor, MI); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kits were from Trevigen Inc. (Gaithersburg,MD); the active form of the caspase −3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); antibodies for phospho-CREB (ser133), CREB, and β-actin were from Cell Signaling Technology (Beverly, MA); nitrocellulose membranes and protein assay kits were from Bio-Rad (Hercules, CA); CREB ShortCut® siRNA, scramble sequence of siRNA and transfection reagents were from New England Biolabs (Ipswich, MA); anti-GPR30, anti-Gαs, shGαs lentiviral particles, and GPR30 siRNA (rats) were from Santa Cruz Biotechnology (Santa Cruz, CA); a reporter plasmid containing a promoter region of the Bcl-2 gene linked to a firefly luciferase gene (Bcl-2-Luc) was a gift of Dr. Linda M. Boxer, Stanford University); a reporter plasmid containing multiple copies of a consensus cAMP responsive element (CRE)-binding sequence fused to a TATA-like promoter region upstream of the gene for firefly luciferase (CRE-luc) was from BD Clontech, CA; the plasmid transfection reagent was from Invitrogen, CA; the ImmPRESS anti-rabbit Ig (peroxidase) polymer detection kit, vector NovaRED peroxidase substrate, and vector SG peroxidase substrate were from Vector Laboratories (Burlingame, CA).

    Labeling:

    Article Title: Discordant growth of nasal cartilage and bone contributes to phenotypic variability of the skull in a mouse model.
    Article Snippet: .. The secondary antibody (ImmPRESS Anti-Rabbit Ig Peroxidase Polymer Detection Kit, Vector Laboratories, Cat. No. MP-6401) for each sample of Osx was labeled with diaminobenzidine (DAB Peroxidase Substrate Kit, 3,30-diaminobenzidine, Vector Laboratories, Cat. No. SK-4100) following standard protocols and counterstained using Methyl Green (Sigma, Cat. No. 67060).17 Osteoclast number was assessed through TRAP staining (Sigma-Aldrich, Cat. No. 387-1KT) as per manufacturer's instructions. ..

    High Performance Liquid Chromatography:

    Article Title: Baicalein Protects against Type 2 Diabetes via Promoting Islet β -Cell Function in Obese Diabetic Mice
    Article Snippet: .. Baicalein (98% pure by HPLC) for in vivo studies was purchased from Xi'An Yile Bio-Tech Company, China; ultrasensitive rat insulin enzyme-linked immunosorbent assay (ELISA) kits were obtained from Mercodia (Winston-Salem, NC); the active form of the caspase-3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); the ImmPRESS Anti-rabbit Ig (peroxidase) Polymer Detection kit, Vector NovaRED peroxidase substrate kit, and Vector SG peroxidase substrate kits were from Vector laboratories (Burlingame, CA); cell viability assay kits were from Promega (Madison, WI); and the BrdU ELISA kit for the cell proliferation assay was from Roche Applied Sciences (Indianapolis, IN). ..

    In Vivo:

    Article Title: Baicalein Protects against Type 2 Diabetes via Promoting Islet β -Cell Function in Obese Diabetic Mice
    Article Snippet: .. Baicalein (98% pure by HPLC) for in vivo studies was purchased from Xi'An Yile Bio-Tech Company, China; ultrasensitive rat insulin enzyme-linked immunosorbent assay (ELISA) kits were obtained from Mercodia (Winston-Salem, NC); the active form of the caspase-3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); the ImmPRESS Anti-rabbit Ig (peroxidase) Polymer Detection kit, Vector NovaRED peroxidase substrate kit, and Vector SG peroxidase substrate kits were from Vector laboratories (Burlingame, CA); cell viability assay kits were from Promega (Madison, WI); and the BrdU ELISA kit for the cell proliferation assay was from Roche Applied Sciences (Indianapolis, IN). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Baicalein Protects against Type 2 Diabetes via Promoting Islet β -Cell Function in Obese Diabetic Mice
    Article Snippet: .. Baicalein (98% pure by HPLC) for in vivo studies was purchased from Xi'An Yile Bio-Tech Company, China; ultrasensitive rat insulin enzyme-linked immunosorbent assay (ELISA) kits were obtained from Mercodia (Winston-Salem, NC); the active form of the caspase-3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); the ImmPRESS Anti-rabbit Ig (peroxidase) Polymer Detection kit, Vector NovaRED peroxidase substrate kit, and Vector SG peroxidase substrate kits were from Vector laboratories (Burlingame, CA); cell viability assay kits were from Promega (Madison, WI); and the BrdU ELISA kit for the cell proliferation assay was from Roche Applied Sciences (Indianapolis, IN). ..

    Viability Assay:

    Article Title: Baicalein Protects against Type 2 Diabetes via Promoting Islet β -Cell Function in Obese Diabetic Mice
    Article Snippet: .. Baicalein (98% pure by HPLC) for in vivo studies was purchased from Xi'An Yile Bio-Tech Company, China; ultrasensitive rat insulin enzyme-linked immunosorbent assay (ELISA) kits were obtained from Mercodia (Winston-Salem, NC); the active form of the caspase-3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); the ImmPRESS Anti-rabbit Ig (peroxidase) Polymer Detection kit, Vector NovaRED peroxidase substrate kit, and Vector SG peroxidase substrate kits were from Vector laboratories (Burlingame, CA); cell viability assay kits were from Promega (Madison, WI); and the BrdU ELISA kit for the cell proliferation assay was from Roche Applied Sciences (Indianapolis, IN). ..

    Proliferation Assay:

    Article Title: Baicalein Protects against Type 2 Diabetes via Promoting Islet β -Cell Function in Obese Diabetic Mice
    Article Snippet: .. Baicalein (98% pure by HPLC) for in vivo studies was purchased from Xi'An Yile Bio-Tech Company, China; ultrasensitive rat insulin enzyme-linked immunosorbent assay (ELISA) kits were obtained from Mercodia (Winston-Salem, NC); the active form of the caspase-3 antibody was from BD Biosciences (San Jose, CA); the rabbit polyclonal anti-insulin antibody was from Abcam (Cambridge, MA); the ImmPRESS Anti-rabbit Ig (peroxidase) Polymer Detection kit, Vector NovaRED peroxidase substrate kit, and Vector SG peroxidase substrate kits were from Vector laboratories (Burlingame, CA); cell viability assay kits were from Promega (Madison, WI); and the BrdU ELISA kit for the cell proliferation assay was from Roche Applied Sciences (Indianapolis, IN). ..



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